糖皮质激素受体__(期刊论文)

TNFα对人糖皮质激素受体表达的影响

http://www.100md.com 《第四军医大学学报》 2005年第17期

糖皮质激素受体,,肿瘤坏死因子α；外周血单个核细胞；糖皮质激素受体,0引言,1材料和方法,2结果,3讨论,【参考文献】

     Effect of TNFα on expression of human glucocorticoid receptors

    ZHAO YanHong, ZHOU Jin, JIA ZhiYan, ZHANG Hua

    1Department of Hematology, 2Ophthalmology Hospital, First Hospital, 3Department of Immunology, School of Basic Medicine, Haerbin Medical University, Haerbin 150001, China

    【Abstract】 AIM: To study the effect of TNFα on the expression of human glucocorticoid receptor (GR) so as to explore the relationship of inflammatory cytokines and glucocorticoid resistance. METHODS: Human peripheral blood mononuclear cells (PBMCs) were isolated and the mRNA expression of GRα and GRβ in PBMC after the culture with TNFα or without TNFα was detected by RTPCR. RESULTS: No significant difference was found between the expression of GRα mRNA in PBMCs with a 24hour TNFα stimulation and that without TNFα stimulation (P>0.05) and GRα/GAPDH mRNA was 1.16±0.73 and 1.20±0.51 respectively. But the expression of hGRβ mRNA in PBMCs with a 24hour TNFα stimulation was significantly higher than that without TNFα stimulation (P<0.05). GRβ/GAPDH mRNA was 1.62±0.59 and 0.81±0.66 respectively. CONCLUSION: TNFα can increase the expression of glucocorticiod receptor β, which may be one of the mechanisms of glucocorticoid resistance induced by inflammatory cytokine. ......

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