Effects of renal ischemic preconditioning on the expressions of Bclª²2, Bax, Fas proteins in immature myocardial cells in rabbits

    SUN Zhongª²Dong, GAO Shangª²Zhi, CHI Yiª²Fan, XIA Jiaª²Hong, YANG Chenª²Yuan

    1Department of Cardiovascular Surgery, Qingdao Municipal Hospital, Medical College, Qingdao University, Qingdao 266011, China, 2Department of Thoracic & Cardiovascular Surgery, People¬ðs Hospital, Wuhan University, Wuhan 430060, China, 3Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China

    ¡¾Abstract¡¿ AIM: To investigate the effects of renal ischemic preconditioning (RIP) on apoptosis and expressions of Bclª²2, Bax and Fas proteins in immature myocardial cells in rabbits. METHODS: Isolated working rabbit heart model was used in this study and 24 immature rabbits were randomly divided into control group, ischemia/reperfusion (I/R) group and RIP group. The TUNEL, nucleosomal ladder of DNA fragments and the expression of Bclª²2, Bax and Fas proteins were detected. RESULTS: Compared with that in I/R group, TUNEL cell apoptosis was lower [(4.19¡À1.13)% vs (12.30¡À2.10)%, P<0.01], ladder of DNA fragments was lower (57 350¡À1765 vs 135 200¡À3370, P<0.01), Bclª²2 expression was higher (33 525¡À1026 vs 12 385¡À860, P<0.01), Bax expression was lower (8640¡À768 vs 32 472¡À1128, P<0.01) and Fas expression was lower (8936¡À912 vs 32 100¡À1260, P<0.01) in RIP group. CONCLUSION: RIP decreases immature myocardial cell apoptosis and affects the expressions of Bclª²2, Bax and Fas proteins. ......