细胞凋亡__(期刊论文)

熊果酸通过影响Bax 和Bcl2的表达诱导MCF7人乳腺癌细胞凋亡

http://www.100md.com 《第四军医大学学报》 2005年第20期

细胞凋亡,,熊果酸；MCF7细胞；细胞凋亡；基因,Bax；,基因,Bcl2；细胞色素C类,熊果酸通过影响Bax和Bcl2的表达诱导MCF7人乳腺癌细胞凋亡,0引言,1材料和方法,2结果

     Ursolic acid induces apoptosis through effects on Bax and Bcl2 expression in human MCF7 cells

    ZHANG GuiPing, LI YinYan, LJiaChun, OU HuiJian

    1Department of Pharmacology, 2Department of Cell Biology, 3Institute of Chemical Carcinogenesis, Guangzhou Medical College, Guangzhou 510182, China

    【Abstract】 AIM: To study the effects of ursolic acid (UA) on human MCF7 cell apoptosis and the mechanism probably involved. METHODS: MCF7 cells were treated with different concentrations of UA (0, 10, 30, 50 μmol/L ) for 24 h, apoptotic cells with condensed or fragmented nuclei were visualized by Ho33258 staining in a fluorescence microscope. After MCF7cells treated with UA (0, 20, 50 μmol/L ) for 24 h, subG1 peak and cell cycle were detected with PI staining by FCM. After MCF7cells treated with and 50 μmol/L of UA for 24 h, the protein expression of cytochrome c, Bcl2 and Bax were detected by immunofluorescence cell staining (SABCCy3) and the grey levels of immunofluorescence on cytochrome c and Bcl2 and Bax were analyzed with the use of QWin. RESULTS: Twentyfour hours after UA treatment, apoptotic cells increased dosedependently and the morphological changes of MCF7 cells displayed many hallmark features of apoptosis, including chromatin aggregation and fragmented nuclei. SubG1 rates in 0, 20 and 50 μmol/L of UA detected by FCM were 0.05, 0.22 and 0.43 respectively. Compared with 5 mL/L DMSO, 50 μmol/L of UA significantly increased the grey levels of Bax (59.3±7.8, 213.6±7.4, P<0.01), cytochrome c (88.2±6.9, 188.1±15.4, P<0.01) and the grey ratio of Bax/Bcl2 (1.0±0.1, 2.4±0.4, P<0.01) and increased noticeably the grey level of Bcl2 (58.1±6.1, 92.1±12.4, P<0.01). Cytochrome c induced by 50 μmol/L of UA released into cytoplasm. CONCLUSION: Apoptosis induced with UA in MCF7 cells is related to increasing the ratio of Bax/bcl2 and releasing cytochrome cdependent apoptotic pathway. Our study indicates that UA may be a potential Chinese medicinal component for breast neoplasm. ......

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