Establishment of serum pharmacology testing method for Compound Realgar Natural Indigo Tablets

    XIANG Yang£¬ CHEN Nanª²Nan£¬ ZHANG Deª²Jie£¬HUANG Shiª²Lin£¬LI Yueª²Meng

    Department of TCM & Hematology, 210th Hospital of Chinese PLA, Dalian 116021, China

    ¡¾Abstract¡¿ AIM: To establish a serum pharmacology testing method for Compound Realgar Natural Indigo Tablets (CRNIT), a traditional Chinese medicine. METHODS: Rabbits were used as the provider to prepare the blood serum with the CRNIT and NB4 cells and K562 cells were the observational objects. The factorial design was applied to observe the effect of medicine administration, deactivation, concentration of blood serum and culture time and the inhibition rates of the leukemia cell lines and to study the interaction between the four factors. RESULTS: ¢Ù The concentration of arsenic in the normal blood serum and the blood serum with medicine from the rabbits was( 0.010¡À0.001) mg/L and (0.110¡À0.006) mg/L respectively (P<0.01). ¢ÚThere were statistical differences between the different levels of each factor. ¢ÛThe different combined blood serum from rabbits with the concentration from 10% to 40% had different inhibitory effect on the growth of NB4 cells and K562 cells in an inhibitory rate and concentration dependent manner. CONCLUSION: ¢Ù 0.75 g/(kg¡¤d) for 5 d is a relatively ideal scheme for preparing the blood serum with CRNIT in rabbits. ¢Ú Four key factors for establishing the reactive system of the blood serum from rabbits with CRNIT are medicine administration or not, deactivation or not, concentration of blood serum and culture time. ¢Û To inactivate the normal blood serum from rabbits can reduce the influence of healthy or pathologic conditions on the experimental and the deactivated blood serum from rabbits can display the antiª²leukemia effect of CRNIT. ¢Ü The experimental result is stable when the concentration of the blood serum ranges from 100 mL/L to 400 mL/L in the reactive system. ......