Study on diacylglycerol acyltransferase 1 gene K378N polymorphism in type 2 diabetes mellitus and diabetic nephropathy

    LIU Haiª²Long, JIANG Hua, ZHANG Ju, JIAO Kai

    1Department of Endocrinology, Tangdu Hospital, Fourth Military Medical University, Xi¬ðan 710038, China£¬2Institute of Genetic Diagnosis, Fourth Military Medical University, Xi¬ðan 710033, China

    ¡¾Abstract¡¿ AIM: To investigate whether diacylglycerol acyltransferase 1 (DGAT1) gene K378N polymorphism relates to Chinese type 2 diabetes mellitus(T2DM) and diabetic nephropathy (DN). METHODS: Gene K378N polymorphism in the DGAT1 gene was genotyped by templateª²directed dyeª²terminator incorporation with fluorescence polarization (TDIª²FP) in 71 patients with T2DM, including 29 patients with DN (DN+) and 42 without DN (DN-), and 45 normal controls. RESULTS: In the T2DM group, there were 42 carriers of DGAT1 KK genotype, 40 of KN genotype and 12 of NN genotype. In the DN+ group, there were 14 carriers of DGAT1 KK genotype, 11 of KN genotype and 4 of NN genotype. In the DN- group, there were 22 carriers of DGAT1 KK genotype, 14 of KN genotype and 6 of NN genotype. In the control group, there were 13 carriers of DGAT1 KK genotype, 23 of KN genotype and 9 of NN genotype. The frequency of DGAT1 K378 allele increased in patients with T2DM but there was no significant difference compared with that in normal controls(P>0.05). There was no significant difference of DGAT1 K378N alleles and genotypes between DN+ and DN- groups(P>0.05). CONCLUSION: DGAT1 K378N may not be associated with the onset of T2DM or DN in Chinese population. ......