Establishment of human osteosarcoma cell sublines with different potential of metastasis and studies on their biological characteristics

    CHEN Xiang, YANG Tongª²Tao, MA Baoª²An, FAN Qingª²Yu

    PLA Orthopedic Oncology Institute, Tangdu Hospital, Fourth Military Medical University, Xi¬ðan 710038, China

    ¡¾Abstract¡¿ AIM: To get human osteosarcoma cell subclones with different potential of metastasis. METHODS: We got different monocell clones by the limited dilution method from the human osteosarcoma cell line SOSPª²9607. After preliminary screening by in vitro invasion assay, we selected two cell lines as high metastasis potential cell subclones and three cell subclones as low metastasis potential cell subclones. We inoculated these subclones into nude mice for three circles and obtained different metastatic potential subclones. We then inspected their different biological properties by tests of cell growth curve assay, chromosome number, soft agar cloning technique, flow cytometry and auto metastasis in nude mice. RESULTS: We got the two subclones with the different metastasis potential: low potential subclone H9 and high potential subclone E10. They had the same hypotriploid karyotype with human chromosome morphology. E10 had faster growth speed than H9 and the artificial lung metastasis rate of H9 and E10 in nude mice was 10% and 100%, respectively. The rate of S phase cell cycle was respectively 16.8% and 21.0%. CONCLUSION: These two subclones come from the same mother cell line and their difference mainly lies in the different metastatic phenotype. This study will help the screening of metastasis correlated gene, the study of the metastatic mechanism and clinical study of osteosarcoma. ......