p38MAPK信号传导通路调控VEGF诱导肝癌细胞黏附作用
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毛 华, 黄纯炽, 赵敏芳, 宋卫生
内皮细胞生长因子;肝癌;HepG2细胞;黏附;转移毛华,黄纯炽,赵敏芳,宋卫生.p38MAPK信号传导通路调控VEGF诱导肝癌细胞黏附作用. 世界华人消化杂志2006;14(8)778-7
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毛华, 黄纯炽, 赵敏芳, 宋卫生, 南方医科大学珠江医院消化科 广东省广州市 510280
毛华, 男, 1963-06-28生, 江西安义县人, 汉族, 2000年第一军医大学博士, 教授, 主要从事消化系统疾病诊疗、消化道肿瘤化疗工作.
通讯作者: 毛华, 510280, 广东省广州市工业大道253号, 南方医科大学珠江医院消化内科. huam@fimmu.com
电话: 020-61643170 传真: 020-84352197
收稿日期: 2005-08-29 接受日期: 2005-09-06
Role of p38MAPK signal transduction pathway in adhesion of hepatocellular cancinoma cell line HepG2 induced by vascular endothelial growth factor
Hua Mao, Chun-Zhi Huang, Min-Fang Zhao, Wei-Sheng Song
Hua Mao, Chun-Zhi Huang, Min-Fang Zhao, Wei-Sheng Song, Department of Gastroenterology, Zhoujiang Hospital of Southern Medical University, Guangzhou 510280, Guangdong Province, China
Correspondence to: Dr. Hua Mao, Department of Gastroenterology, Zhoujiang Hospital of Southern Medical University, Guangzhou 510280, Guangdong Province, China.huam@fimmu.com
Received: 2005-08-29 Accepted: 2005-09-06
Abstract
AIM: To investigate the role of the p38MAPK signal transduction pathway in the metastasis and adhesion of hepatocellular carcinoma cell line HepG2 induced by vascular endothelial growth factor (VEGF).
METHODS: Hepatocellular cancinoma cell line HepG2 were or not pretreated with specific blocker (SB203580) of p38MAPK signal transduction pathway. 3H-TdR infiltration and the experiment of rat tail colloid were used to measure the effects of different concentrations of VEGF on the homotypic and hetertypic adhesion in HepG2 cells. Flow cytometry was adopted to detect VEGF-induced expression of CD44v6 and by Boyden-Chamber assay was used to evaluate VEGF-induced metastasis of HepG2 cells.
RESULTS: After 1 and 5 mg/L VEGF induction for 60 min, the values (dpm/min) of 3H-TdR infiltration in HepG2 cells were 1 758.67 ± 289.46 and 1 380.03 ± 328 ......
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