siRNA对生长抑素基因表达的抑制作用
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朱晓燕, 岳保红, 张钦宪
生长抑素;RNA干扰朱晓燕,岳保红,张钦宪.siRNA对生长抑素基因表达的抑制作用. 世界华人消化杂志2006;14(8)784-788,朱晓燕,张
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朱晓燕, 张钦宪, 郑州大学医学院组胚教研室 河南省郑州市450052
岳保红, 郑州大学第一附属医院检验科 河南省郑州市 450052
朱晓燕, 女, 1976-12-26生, 在读博士, 郑州大学医学院组胚教研室讲师, 主要从事消化道肿瘤分子生物学研究.
通讯作者: 张钦宪, 450052, 河南省郑州市, 郑州大学医学院组胚教研室. qxz53@zzu.edu.cn
电话: 0371-66658153 传真: 0371-66658153
收稿日期: 2005-12-21 接受日期: 2006-02-25
Inhibitory effects of small interfering RNA on expression of somatostatin gener
Xiao-Yan Zhu, Bao-Hong Yue, Qin-Xian Zhang
Xiao-Yan Zhu, Qin-Xian Zhang, Department of Histology and Embryology, Medical College of Zhengzhou University, Zhengzhou 450052, He’nan Province, China
Bao-Hong Yue, Department of Clinical Laboratory, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, He’nan Province, China
Correspondence to: Dr. Qin-Xian Zhang, Department of Histology and Embryology, Medical College of Zhengzhou University, Zhengzhou 450052, He’nan Province,China. qxz53@zzu.edu.cn
Received: 2005-12-21 Accepted: 2006-02-25
Abstract
AIM:To research inhibitory effects of small interfering RNA (siRNA) on the expression of somatostatin gene.
METHODS:According to the gene sequence of somatostatin in GenBank, we designed the siRNA-targeted templates and synthesized siRNA using T7 RiboMAX Express RNAi System in vitro. The obtained siRNA was then transfected into gastric cancer cell line SGC-7901. Reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemical techniques were used to detect the expression of somatostatin at both mRNA and protein levels.
RESULTS:The target siRNA with a length of 21 bp was successfully synthesized. Before transfection, somatostatin was strongly and positively expressed in gastric cancer cell line SGC-7901, locating at the cytoplasm. Forty-eight hours after transfection , somatostatin expression was markedly inhibited and the inhibitory rate in siRNA-transfected cells was significantly higher than that in the cells transfected with empty vector and non-transfected cells (49 ......
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