关键词: 微波;p27;癌，肝细胞

    摘 要:目的 观察2450MHz微波对人肝癌细胞p27mRNA表达的影响，以探讨其抑制肝癌细胞存活的分子机制. 方法 转染p27基因的人肝癌细胞按微波辐照强度不同分为对照组(未辐照组)、10mW·cm-2 ，20mW·cm-2 和30mW·cm-2 组，分别于微波屏蔽室内辐照1h后，用四唑盐比色试验(MTT)检测微波对肝癌细胞的抑制作用;同时用RT-PCR方法检测肝癌细胞中p27mRNA的表达，并进行灰度扫描后计算RNA的表达指数. 结果 微波辐照1h对肝癌细胞存活有明显的抑制作用，且呈剂量-效应关系.微波可以上调肝癌细胞中p27mRNA的表达，且与微波辐照强度有剂量-效应关系. 结论 微波可能通过上调p27mRNA的表达，从而抑制肝癌细胞的存活.

    Effects of microwave radiation on the expression of p27mRNA in human hepatocellular carcinoma cells

    LUO Wen-Jing WANG Wen-Liang REN Dong-Qing CHEN Jing-Yuan WANG Feng CHEN Yao-Ming

    1 Department of Occupational&Environmental Hy-giene，Faculty of Preventive Medicine，

    2 Department of Pathology，Faculty of Preclinical Medicine，

    3 Department of Radiation Medicine，Fourth Military Medical University，Xi'an710033，China

    Keywords:microwaves;p27;carcinoma，hepatocellular

    Abstract:AIM To observe the expression of p27mRNA in hepatocellular carcinoma cell so as to study the proliferative mechanisms of microwave radiation on hepatocellular carcino-ma cell.METHODS A hepatocellular carcinoma cell strain which can express p27protein stably was divided into control(non-irradiation) ......