关键词: 螺杆菌，幽门;粘附素alpA;克隆，分子;基因表达

    摘 要:目的 克隆并表达幽门螺杆菌粘附素AlpA基因. 方法 提取幽门螺杆菌染色体基因，用PCR方法扩增alpA基因，将其克隆至表达载体pET-22b(+)，转化大肠杆菌BL21(DE3)，IPTG诱导表达. 结果 分离得到了1.5kb的alpA基因片段，并在大肠杆菌中实现了该基因的高效表达，在37℃诱导表达3h后，表达产物占细菌总蛋白的31.9%.表达以可溶性蛋白和包涵体两种形式存在，其中主要是包涵体的形式，目的蛋白占不溶性蛋白的64.8%. 结论 幽门螺杆菌alpA基因的克隆与表达为Hp疫苗的研制打下了基础.

    Cloning and high expression of Helicobacter pylori adhesion alpA gene

    BAI Yang ZHANG Ya-Li WANG Ji-De YANG Yun-Sheng ZHANG Zhao-Shan ZHOU Dian-Yuan

    1 Institute for Digestive Medicine of Chinese PLA，First Military Medical University，Guangzhou510515，China;

    2 Department of Digestive Medicine，301Hospital of Chinese PLA，Beijing100853，Chi-na;

    3 Institute of Biotechnology，Academy of Mili-tary Medical Sciences，Beijing100071，China

    Keywords:helicobacter pylori;adhesion alpA;cloning mole-cular;gene expression

    Abstract:AIM To isolate and expresse alpA gene from He-licobacter pylori.METHODS The alpA gene was amplified from H.pylori chromosomal by PCR and inserted into the prokaryotie expression vector pET-22b(+).The recombinant plasmid was transformed into the BL21(DE3)E.coli strain.RESULTS 1.5kb alpA gene was successfully isolated.Re-combinant E.coli strains expressed AlpA were obtained ......