关键词: 前庭终器;脱噬作用;生长和胚胎发育

    摘 要:目的 研究豚鼠胚胎正常发育期前庭终器形态变化及细胞凋亡，探讨细胞凋亡在前庭终器发育中的作用. 方法 选用胚胎40和50d正常豚鼠，光镜观察其前庭终器发育形态变化，末端脱氧核苷酸转移酶介导的生物dUTP缺口末端标记技术(TUNEL)测定毛细胞和支持细胞凋亡，并以电镜观察凋亡细胞胞核变化. 结果 胚胎40d，豚鼠前庭壶腹、椭圆囊斑及球囊斑已分化，前庭上皮出现一层毛细胞及多层支持细胞;胚胎50d，支持细胞层数减少，毛细胞数目增加，伴有神经纤维出现.TUNEL检测提示大量毛细胞和支持细胞发生凋亡.电镜下前庭上皮细胞发生分化，凋亡的毛细胞和支持细胞胞核染色质浓缩边集.凋亡细胞与新生细胞相邻. 结论 细胞凋亡是豚鼠内耳前庭终发育的必然现象，凋亡的发生对保证内耳正常结构和功能密切相关.

    Development and cell apoptosis of vestibular end or┐gans in embryonic guinea pig

    HU Jin-Wei，HUANG Wei-Guo，JIANG Hong-Yan，WANG Jin-Ling，LIU Shun-Li，QIU Jian-Hua

    Department of Otolaryngology，Xijing Hospital，Fourth Military Medical University，Xi'an710033，China

    Keywords:vestibular end organs;apoptosis;grouth and em-bryonic development

    Abstract:AIM The morphological changes and cell apopto-sis of vestibular organ in normally developing guinea pigs were observed to elucidate the effect of apoptosis in the de-veloping vestibular organ.METHODS Guinea pigs of em-bryonic day(ED)40and50were studied.The morphological changes in the vestibular organ were observed by light micro-scope and apoptosis by TdT-mediated deoxyuridine triphos-phate-biotin nick end labelling(TUNEL)method and trans-mission electro microscopy(TEM).RESULTS Ampullary cristae or macula of saccule and utricle were well differentiat-ed in ED40.There were a layer of hair cells and several lay-ers of supporting cells.The number of hair cells increased and that of layers of supporting cells decreased ......