关键词: 高氧液;脑缺血;再灌注损伤;自由基;一氧化氮

    摘 要:目的 探讨高氧液对兔全脑缺血再灌注损伤的保护作用. 方法 18只家兔随机分3组(n=6).用夹闭双侧颈总动脉加降压法建立全脑缺血模型.A组再灌注时用平衡液20mL kg-1 静脉推注(对照组);在再灌注时用高氧液20mL kg-1 静脉推注(治疗组);C组于实验前3d用高氧液20mL kg-1 静脉推注每日1次(预处理组).各组在缺血前、缺血20min和再灌注45min抽取颈内静脉血测定血浆一氧化氮(NO)，超氧化物歧化酶(SOD)和丙二醛(MDA). 结果 再灌注后B组和C组MDA含量明显低于A组(P<0.01或P<0.05)，B组和C组血浆SOD活性高于A组(P<0.05).B组和C组血清NO含量明显低于A组(P<0.01或P<0.05). 结论 高氧液对神经元缺血损伤具有保护作用.

    Protective effect of hyperoxia solution on brain in┐jury resulting from ischemia and reperfusion in rab┐bits

    ZENG Zhen XU Li-Xian LI Ren

    1 Department of Anesthesiology，Stomataological College，

    2 Department of Hepatobiliary Surgery，Xijing Hospital，Fourth Military Medical Universi-ty，Xi'an710033，China

    Keyword:hyperoxia solution;brain ischemia;reperfusion in-jury;free radical;nitric oxide

    Abstract:AIM To investigate the protective effect of hy-peroxia solution on the cerebral ischemia model.METHODS 18domestic rabbits were randomly divided into three groups.In Group A the balance salt20mL kg-1 (which was replaced by hyperoxia solution in Group A)was given intra-venously to the animals after reperfusion.And in Group C hyperoxia solution20mL kg-1 was infused intravenously ev-eryday for3d before ischemia.The amount of NO and MDA as well as the activity of SOD in plasma were observed at dif-ferent time points(before ischemia ......