关键词: myc基因;反义核酸;大鼠;平滑肌

    摘 要:目的 观察反义c-myc寡核苷酸对大鼠气道平滑肌增殖的抑制作用. 方法 大鼠气道平滑肌细胞原代培养，4～12代用于实验.利用Lipofectin将正义、反义及错配c-myc寡核苷酸导入大鼠气道平滑肌细胞，MTT法检测不同寡核苷酸对大鼠气道平滑肌细胞增殖的抑制作用，RT-PCR检测c-myc mRNA的表达，免疫组织化学染色检测c-Myc蛋白的表达. 结果 反义c-myc寡核苷酸可抑制大鼠气道平滑肌细胞增殖，且这种抑制作用呈浓度依赖性;反义c-myc寡核苷酸可降低c-myc mRNA的表达，同时降低了c-myc mRNA的翻译. 结论 反义c-myc寡核苷酸可抑制大鼠气道平滑肌的增殖.

    Antiproliferative effect of c┐myc antisense oligonu┐cleotide on rat airway smooth muscle cells

    LIU Ying-Ge QI Hao-Wen LI Huan-Zhang SU Ming-Quan YU Wen-Bin MA Yue-Yun

    1 Department of Respiratory Disease，

    2 Department of Clinical Laboratories，Xijing Hospital，Fourth Mili-tary Medical University，Xi'an710033，China

    Keywords:genes，myc;oligonucleotide，antisense;rat;muscle，smooth

    Abstract:AIM To observe the antiproliferative effect of c-myc antisense oligonucleotide on rat airway smooth muscle cells.METHODS Rat airway smooth muscle cells were pri-mary cultured.4～12passages were used in experiments.Lipofectin was used to transfect antisense oligonucleotide，sense oligonucleotide and mismatched oligonucleotide for c-myc to rat airway smooth muscle cells.The antiproliferative effect was assayed by way of MTT.RT-PCR was used to de-tect the expression of c-myc mRNA.c-Myc was observed by immunohistochemistry staining.RESULTS Antisense oligonucleotide for c-myc could inhibit the proliferation of rat airway smooth muscle cells ......