关键词: 幽门螺杆菌;尿素酶B基因;大肠杆菌

    摘 要:目的 克隆幽门螺杆菌ureB基因，并进行表达，验证表达产物的反应原性. 方法 应用PCR技术从临床分离株中扩增出ureB基因，克隆入载体pGEM-7zf(+)进行测序，进一步转到大肠杆菌表达载体pBV220进行诱导表达，以Western blot实验验证重组蛋白的反应原性. 结果 测序后经同源性比较，证实扩增后得到的片断确为ureB基因，并原核表达出可被抗Hp抗体识别的非融合重组蛋白. 结论 获得了有反应原性的重组UreB蛋白.

    Cloning and expression of Helicobacter pylori ureB and its reactionogenicity analysis

    QIN Li-Peng，LI Ding，ZHANG Ju，HAN Feng-Chan，YAN Xiao-Jun

    Institute of Genetic Diagnosis of Chinese PLA，Fourth Military Medical University，Xi'an710033，China

    Keywords:Helicobacter pylori;urease B gene;E.coli

    Abstract:AIM To clone and to express the ureB gene of Helicobacter pylori and analyse the reactionogenicity of the recombinant ureB.METHODS H.pylori ureB fragments were amplified by PCR ......