关键词: 毛细胞，前庭;蛋白激酶C;器官培养;佛波酯

    摘 要:目的 观察体外培养条件下蛋白激酶C(protein ki-nase C，PKC)在佛波酯(phogrbol12-myristate13-acetate，PMA)诱导下大鼠前庭上皮的表达和分布特点. 方法 取新生大鼠椭圆囊，建立体外前庭器官培养模型，采用免疫组织细胞化学的方法，以PKCβ，γ抗体为标记物，检测PMA诱导后前庭上皮PKCβ，γ的表达变化和分布特点. 结果 PKCβ，γ在正常培养新生大鼠前庭上皮细胞的细胞膜及细胞质中有弱表达.PMA作用后，其表达逐渐增多，PKCβ1 主要位于细胞膜，在30min时表达最强，其后逐渐减少，45min仍高于正常(P<0.01).PKCβ2 不但位于细胞膜，而且在纤毛亦明显表达;PKCγ在PMA诱导后胞质表达增加.在毛细胞层和支持细胞层均有，主要位于毛细胞层. 结论 体外前庭器官培养在PMA诱导后，PKC各亚型激活的形式及程度不同.

    Expression of PKC in vestibular epithelium of rat during organ culture in vitro

    SUN Jing-Yu，HUANG Wei-Guo，WANG Jin-Ling，QIU Jian-Hua，JIANG Hong-Yan

    Department of Otorhinolaryngology，Xijing Hospi-tal，Fourth Military Medical University，Xi'an710033，China

    Keywords:hair cells，vestibular;protein kinase C;organ culture;phogrbol12-myristate13-acetate

    Abstract:AIM To investigate the expression of PKCβ，γof vestibular epithelium in rats after PMA treatment during or-gan culture in vitro so as to elucidate the biological character.METHODS The vestibular organ was placed in the bottom of a culture plate which contained culture medium to grow.After the initial culture period of24h ......