关键词: 癌，鳞状细胞;肺肿瘤;人乳头瘤病毒;p53基因

    摘 要:目的 探讨肺鳞癌组织中人乳头瘤病毒(HPV)感染与p53蛋白表达的关系并分析作用机制. 方法 收集84例肺鳞癌和18例支气管粘膜慢性炎症标本，采用地高辛标记的HPV-DNA探针，用原位杂交法检测组织中的HPV-DNA存在情况，并用免疫组化SABC法检测肺鳞癌组织中p53及HPVE6，E7蛋白的表达，将二者进行对照观察. 结果 ①HPV原位杂交阳性信号位于细胞核内，肺鳞癌HPV检出率为49%，对照组慢性炎为6%，差异显著.②肺鳞癌组织中存在HPVE6，E7蛋白表达，免疫组化方法与原位杂交方法结果相符.③84例肺鳞癌中30例检出p53蛋白表达，其中17例HPV阳性，13例HPV阴性，HPV阳性组与阴性组差异不显著. 结论 肺鳞癌与HPV感染关系密切，肺鳞癌组织中也存在p53基因表达，p53蛋白积聚与HPV感染无明显相关性.

    Study of human papillomavirus infection in squa┐mous cell carcinoma of lung and the relationship with the accumulation of p53

    CHEN Wei-Qiang，QI Hao-Wen

    Department of Respiratory Disease，Xijing Hospi-tal，Fourth Military Medical University，Xi'an710033，China

    Keywords:carcinoma，squamous cell;lung neoplasms;hu-man papillomavirus;p53gene

    Abstract:AIM To investigate the relationship between hu-man papillomavirus(HPV)infection and the accumulation of p53in squamous cell carcinoma(SQCC)of lungs.METHODS 84of SQCC and18of bronchial chronic inflam-mation samples were detected by in situ hybridization for hu-man papillomavirus using digoxingemin labelled DNA probe.HPV-E6 ......