关键词: 心肌顿抑;脂质体;三磷酸腺苷

    摘 要:目的 观察恢复顿抑心肌的能量代谢对其功能的影响. 方法 在18只麻醉开胸犬上建立局部心肌顿抑(My-ocardium Stunning)模型，在顿抑心肌局部灌注脂质体包裹的三磷酸腺苷(ATP)，用高效液相色谱法测量心肌腺苷酸含量.动态监测血流动力学及局部心肌收缩力的变化. 结果 冠脉灌注脂质体携载ATP可以明显提高心肌细胞ATP含量，减少顿抑局部血清丙二醛的产生，降低心肌细胞Ca2+ 含量，提高局部心肌收缩力，加快心脏收缩功能的恢复.而冠脉灌注游离ATP则无此作用. 结论 顿抑心肌能量代谢与心功能之间有着密切联系，提高顿抑心肌ATP含量可以加快心功能的恢复.脂质体作为载体可以将外源性ATP导入心肌细胞内，是一种很有前途的治疗措施.

    Effects of liposome┐entrapped ATP on Stunned my┐ocardium in dogs

    LI Wei-Jie，JIA Guo-Liang，GUO Wen-Yi，LIU Bing

    Department of Cardiovasology，Xijing Hospital，Fourth Military Medical University，Xi'an710033，China

    Keywords:Myocardium stunning;liposome;ATP

    Abstract:AIM To test whether restoration of adenosine triphosphate(ATP)levels in myocytes after brief coronary artery occlusion might improve the function of postischemic myocardium.METHODS Eighteen anesthetized，ventilated dogs underwent thoracotomy.A silastic perfusion shunt was established between the left carotid and proximal LAD arter-ies.Then the dogs were subjected to15min of LAD occlu-sion and60min of reperfusion.ATP-liposome(n=6，2.5mmol·min-1 )，free ATP(n=6 ......