关键词: 卵巢肿瘤;端粒，末端转移酶;核糖核酸酶类;转染

    摘 要:目的 研究端粒酶RNA特异性核酶对卵巢癌细胞活性的抑制作用. 方法 用脂质体法将构建好的核酶逆转录病毒载体转染至卵巢癌HO-8910细胞，以MTT比色法检测细胞的生长，应用流式细胞仪分析细胞周期细胞，观察细胞的增殖情况，同时扩增端粒重复序列，结合杂交分析，检测细胞的端粒酶活性. 结果 MTT检测发现转染核酶后的细胞生长的潜伏期延长(48→72h)，对数生长期减缓(4→5d)，流式细胞仪结果显示其G1期比例明显增高(70.0%→80.5%，P<0.01)，S期细胞比例明显降低(19.0%→13.7%，P<0.01)，细胞增殖下降，细胞的端粒酶活性亦降低. 结论 端粒酶RNA特异性核酶能抑制卵巢癌细胞端粒酶活性，抑制细胞增殖.

    Attenuation of telomerase activity by a hammerhead ribozyme targeting the template region of telom┐erase RNA in ovarian carcinoma cell

    WANG Hong-Ying XIN Xiao-Yan MA Yue-Yun SU Ming-Quan

    1 Department of Obstetrics& Gynecology，

    2 Laboratory of Clinical Molecular Biology，Xijing Hospital，Fourth Military Medical University，Xi'an710033，China

    Keywords:ovarian neoplasms;telomere，terminal trans-ferase;ribonucleases;transfection

    Abstract:AIM To explore the effects of hammerhead ri-bozyme against human telomerase RNA in ovarian carcinoma cells.METHODS We transfected the ribozyme directed a-gainst the RNA component of human telomerase into HO-8910 ......