关键词: 汉坦病毒;肾综合征出血热;空斑实验;基因重组;聚合酶链式反应

    摘 要:目的 探明汉坦病毒汉滩型与希望山型之间能否发生基因重排，以及发生重排的频率和特点. 方法 用汉坦病毒汉滩型76-118株与希望山型PHV株混合感染Vero E6细胞，空斑形成实验挑取子代病毒克隆株，传代培养后用RT-PCR方法鉴定子代病毒基因型. 结果 子代病毒14/36来源于PHV株;12/36来源于76-118株;5/36汉滩型与希望山型分型引物扩增均为阳性;1株子代病毒L，S片断均来源于76-118株，而M片段两种引物扩增均为阳性;2株子代病毒L片断来源于76-118株，S片断来源于PHV株，M片段两种引物扩增均为阳性.1株子代病毒L，S片断均来源于76-118株，而M片段来源于PHV株. 结论 汉坦病毒汉滩型与希望山型之间可以发生基因片段的重排.

    Genetic reassortment between Hantaan virus strains and prospect hill virus strains

    KANG Wen-Zhen，HUANG Chang-Xing，BAI Xue-Fan，YANG Wei-Song，XIE Yu-Mei，HAO Chun-Qiu，ZHANG Yan

    Department of Infectious Diseases，Tangdu Hospi-tal，Fourth Military Medical University，Xi'an710038，China

    Keywords:hantavirus;recombination，genetic;polymerase chain reaction

    Abstract:AIM To determine the frequency and characteris-tics of gene segments reassortment between Hantaan virus and Prospect Hill virus.METHODS Mixed infections were initiated in tissue culture by using Hantaan virus strains(76-118)and Prospect Hill Virus Strains(PHV).Potential reas-sortant virus plaques were screened by multiplex RT-PCR ......