关键词: 水杨酸钠;HL-60细胞;脱噬作用

    摘 要:目的 研究水杨酸钠对HL-60细胞的影响. 方法 用MTT法检测水杨酸钠对HL-60细胞存活率的影响;光镜、电镜观察细胞形态学变化;流式细胞术分析DNA含量;An-nexin-V结合分析检测靶细胞磷脂酰丝氨酸外化. 结果 水杨酸钠明显抑制HL-60细胞生长，并呈时间、剂量依赖性;5mmol L-1 (IC50 )的水杨酸钠作用后，HL-60细胞出现典型的凋亡形态特征，并检测到亚二倍体峰;同时，靶细胞磷脂酰丝氨酸外化率显著高于阴性对照组(0.24vs0.03). 结论 水杨酸钠能抑制HL-60细胞生长并促进其凋亡.

    Effect of sodium salicylate on human acute promye┐locytic leukemia HL┐60cells

    WAN You-Feng CHEN Xie-Qun LI Yuan LI Bie-Hu

    1 Department of Hematology，Xijing Hospital，

    2 Department of Microbiology，Faculty of Preclinical Medicine，Fourth Military Medical University，Xi'an710033，China

    Keywords:sodium salicylate;HL-60cells;apoptosis

    Abstract:AIM To study the growth-inhibited and apopto-sis-triggered effect of sodium salicylate(Na-Sal)on HL-60cells.METHODS The viability of HL-60cells was mea-sured by MTT assay;the morphological changes were ob-served by light and eletronic microscope;DNA content was assayed by flow cytometry;and the phosphatidylserine(PS)exposure was detected by Annexin-V binding.RESULTS Na-Sal was effective in inhibiting the growth of HL-60cells in a dose-and time-dependent manner.HL-60cells treated by Na-Sal with a concentration of5mmol L-1 (IC50 )were typical of apoptotic morphology ......