逆转录病毒载体介导的端粒酶抑制基因促进肝癌细胞凋亡
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马进财, 刘吉勇, 刘绍玲
重组逆转录病毒载体;基因疗法;肝细胞癌;反义hTR,马进财,刘吉勇,刘绍玲,通讯作者:,ApoptosisofBEL-7402celllinem
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马进财, 刘吉勇, 山东大学山东省立医院消化科 山东省济南市 250021
刘绍玲, 山东省医学影像学研究所 山东省济南市 250021
山东省医药卫生科研项目, No. CAIDBA3
通讯作者: 刘吉勇, 250021, 山东省济南市经五路324号, 山东省立医院消化科. mjcmjc@126.com
收稿日期: 2006-01-19 接受日期: 2006-01-24
Apoptosis of BEL-7402 cell line mediated by retroviral vector containing antisense human telomerase RNA
Jin-Cai Ma, Ji-Yong Liu, Shao-Ling Liu
Jin-Cai Ma, Ji-Yong Liu, Department of Gastroenterology, Shandong Provincial Hospital of Shandong University, Ji’nan 250021, Shandong Province, China
Shao-Ling Liu, Shandong Provincial Medical Image Research Institute, Ji’nan 250021, Shandong Province, China
Correspondence to: Ji-Yong Liu, Department of Gastroenterology, Shandong Provincial Hospital of Shandong University, 324 Jingwu Road, Ji’nan 250021, Shandong Province, China. mjcmjc@126.com
Received: 2006-01-19 Accepted: 2006-01-24
Abstract
AIM: To investigate the apoptosis-inducing effect of retroviral vector containing antisense human telomerase RNA (hTR) on hepatocellular carcinoma (HCC) cell line BEL-7402.
METHODS: The complete virus was obtained by importing the sense and antisense hTR into the PT67 cell line with electroporation. Bel-7402 cell line was then transfected with the virus. The therapeutic effect was evaluated by cell growth curve. MTT assay and flow cytometry were used to investigate the apoptosis of BEL-7402 cells.
RESULTS: After transfection with retroviral vector, the growth of BEL-7402 cells was obviously inhibited. The apoptotic rate was significantly higher in the cells transfected with antisense hTR than that in the cells transfected with sense hTR and normal saline controls (61.32% ± 2.24% vs 23.02% ± 2.13%, 4.11% ± 1.00%, P < 0.01), and the apoptotic rate was more significant when the antisense hTR-transfected cells were treated with 5-FU (71.71% ± 2.53%).
CONCLUSION: hTR can induce significant apoptosis of BEL-7402 cell line, and it plays a synergic role with 5-FU ......
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