[摘要]目的 测定巴洛沙星合成中的主要中间体羧酸乙酯在巴洛沙星中的含量。 方法 反相高效液相色谱法(RP.HPLC)。 结果 样品在0.30～5.96μg·ml -1 范围内线性关系良好，中间体的平均回收率为96.2%，相对标准偏差(RSD)为1.85%。 结论 RP.HPLC法测定准确，重现性良好，可用于巴洛沙星的质量控制。

    [关键词] 反相高效液相色谱法;巴洛沙星;羧酸乙酯

    Assaying of balofloxacin carboxylic acester by RP.HPLC

    YUHong ，DAI Zhen-nan ，MIAO Yu-shan ，SHI Tao

    (Department of Pharmacy，Nanjing720Factory Hospital，Nanjing210037，China;Department of Pharmacy，Zhongda Hospital，Southeast University，Nanjing210009，China)

    Abstract:Objective To determine the content of balofloxacin carboxylic acester during the synthesize of balofloxacin.Methods The content of balofloxacn carboxylic acester was assayed by reverse phase high press liquid chromatography(RP.HPLC).Results There was a good linear relationship within the range of0.30～5.96μg·ml(r=0.99997).The average recovery and relative standard deviation of the method were96.2%and1.85%respectively.Conclusion The method is accurate ......