Changes of autoantibodies against ¦Â1 adrenergic receptor and M2 cholinergic receptor in chronic glomerulonephritis with renal failure

    ZHAO Linª²Shuang1, LIAO Yuª²Hua2, WANG Min2, XIANG Guangª²Da1,HOU Jie1, YUE Ling1£¬ LAN Tianª²Biao3, WANG Xu3,WANG Ping3

    1Department of Endocrinology, 3Department of Nephrology, Wuhan General Hospital, Guangzhou Military Area Command, Wuhan 430070,China, 2Institute of Cardiovascular Diseases,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology, Wuhan 430022, China

    ¡¾Abstract¡¿ AIM: To explore the role of autoantibodies against M2 muscarinic cholinergic receptor(M2 receptor) and ¦Â1 adrenergic receptor(¦Â1 receptor)in the development of chronic glomerulonephritis (CGN) with renal failure. METHODS: The epitopes of the second extracellular loop of M2 receptor (169-191) and ¦Â1 receptor (197-222) were synthesized and used respectively to screen serum autoantibodies from patients with CGN with renal failure (n=66), hypertension patients without renal failure (n=58) and healthy blood donors (n=40, control) by ELISA. RESULTS: In CGN patients with renal failure, the positive rates of the autoantibodies against ¦Â1 receptor and M2 receptor were 62.1% and 48.5% respectively, which were both higher than those in the hypertension patients without renal failure (19.0% and 20.7% respectively) and the healthy donors (17.5% and 15.0% respectively) (P<0.01). CONCLUSION: These autoantibodies against ¦Â1 receptor and M2 receptor may play some important roles in the pathogenesis of CGN with renal failure. ......