Effects of Ginaton on hypoxiaª²induced endothelin secretion from human aortic endothelial cells

    LI Mingª²Gao, MA Guiª²Xi, HAN Lei, LIU Yu, LI Jing, MENG Guo, LIU Xin

    Center of Aerospace and Diving Medicine, Navy General Hospital, Beijing 100037, China

    ¡¾Abstract¡¿ AIM: To explore the effects of Ginaton at different concentrations on the hypoxiaª²induced endothelin (ET) secretion from cultured vascular endothelial cells (VECs). METHODS: Cultured VECs were divided into 5 groups: control group, simple hypoxia group, highª²concentration Ginaton group, moderateª²concentration Ginaton group, and lowª²concentration Ginaton group. For hypoxia, all groups except control group were put in a hypobaric chamber at the height of 5000 m for 30 min. The ET contents in supernatant of VECs before hypoxia and 0.5 h after hypoxia, 6 h after hypoxia as well as 24 h after hypoxia were measured with radioimmunoassay. RESULTS: ¢Ù Simple hypoxia promoted VECs¬ð secretion of ET and ET content increased significantly at 0.5 h after hypoxia and reached the peak at 6 h (P<0.01). ¢Ú Ginaton inhibited VECs¬ð secretion of ET induced by hypoxia. At the time of 0.5 h after hypoxia, highª²concentration Ginaton was more effective than moderateª²concentration and lowª²concentration Ginaton (P<0.01). At the time of 6 h and 24 h after hypoxia, moderateª²concentration Ginaton was more significantly effective than highª²concentration and lowª²concentration Ginaton (P<0.05). CONCLUSION: Ginaton has significant protective effects on hypoxiaª²induced ET secretion from cultured VECs and the effects of moderateª²concentration Ginaton are more obvious compared with those of highª²concentration and lowª²concentration Ginaton. ......