结核杆菌_____]胶体金；免疫层析；结核杆菌

胶体金免疫层析法检测结核杆菌抗体的研究

http://www.100md.com 《海军医学杂志》 2005年第2期

结核杆菌,,]胶体金；免疫层析；结核杆菌,1材料,2方法,3结果,4讨论,[参考文献]

     [摘要]目的：应用S3800作为标记源建立结核杆菌抗体的胶体金免疫层析检测方法。方法：用柠檬酸三钠还原法制备胶体金，免疫层析检测抗体，ELISA法进行符合率检测。结果：建立的胶体金免疫层析检测方法灵敏度达2 mg/L，特异性好，无交叉反应，与ELISA法相比阳性符合率达93.8%，试剂稳定性达6个月。结论：建立的胶体金免疫层析检测方法灵敏度高，特异性好，有广泛的应用价值。

    [关键词]胶体金；免疫层析；结核杆菌

    Goldimmunochromatography assay for mycobacterium tuberculosis

    CHEN Shuanghong, WANG Fasuo, TIAN Li, et al

    (Naval Medical Research Institute, Shanghai 200433，China)

    Abstract: Objective: To develop a sensitive, rapid and simple colloidal gold immunochromatography assay for detecting TBAb in serum. Methods: Colloidal gold was prepared with the trisodium citrate reduction method. TBAb was detected with immunochromatography. Rate of coincidence was compared with ELISA. Results: The study has achieved the following initial results: sensitivity of the established method is 2 mg/L, with good specificity, but without crossreaction; rate of coincidence is as high as 93.8 %, when compared with that of ELISA; and the reagent can be kept as long as 6 months. Conclusion: Gold immunochromatography assay for TBAb is a method that has a wide range of application, high sensitivity and good specificity. ......

百拇医药网 http://www.100md.com/html/DirDu/2006/04/25/96/85/53.htm

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