摘要 :目的 研究经纹状体注射胶质细胞系源性神经营养因子(glial cell line-derived neurotrophic factor，GD-NF)对帕金森病(Parkinson disease，PD)模型小鼠中脑黑质多巴胺能神经元的保护作用。 方法 利用1-甲基-4-苯基1，2，3，6-四氢吡啶(1-methyl-4-phenyl-1，2，3，6-tetrahydropyridine，MPTP)制备成年小鼠PD模型，通过单侧纹状体定位注射GDNF，取中脑黑质节段，做连续冠状石蜡切片，结合免疫组织化学染色方法，观察各组酪氨酸羟化酶(tyrosine hydroxylase，TH)、钙结合蛋白(calbindin D28k，CB)的表达，光镜观察并细胞计数，统计学分析。 结果 在模型制备的第4、6天，单侧纹状体定位注射GDNF后，小鼠黑质致密部TH与CB阳性神经元数量显著多于注射PBS组。 结论 经纹状体注射GDNF对成年PD小鼠黑质多巴胺能神经元具有保护作用，且这种保护作用可能与细胞内CB表达增加有关。

    关键词 :胶质细胞系源性神经营养因子;多巴胺能神经元;1-甲基4-苯基-1，2，3，6-四氢吡啶

    The protective effect of intrastriatal GDNF on the dopaminergic neurons in Parkinson disease mice

    WANG Yan-qiang，LIU Hong-mei，WANG Hong-jun，et al

    (Department of Neurobiology，Xuzhou Medical College，Xuzhou，Jiangsu221002，China)

    Abstract:Objective To investigate the protective effect of intrastriatal GDNF on dopaminergic(DA)neurons in the sub-stantia nigra of MPTP-induced mouse model of Parkinson disease(PD).Methods The PD model was established in adult KM mice four or six days after an intraperitoneal injection of MPTP30mg.kg.The model mice were then treated by giving GD-NF into the striatum.After a seven-day survival period ......