摘要 :目的 克隆宫颈癌MN.CA9抗原的多糖蛋白cDNA，并构建表达载体。 方法 从宫颈癌HeLa细胞中提取总RNA，RT-PCR法扩增MN.CA9抗原的多糖蛋白cDNA，将其插入载体pCA13，转化大肠杆菌JM109，构建MN.CA9抗原的多糖蛋白表达载体pCA13-MN。酶切及测序鉴定。 结果 酶切及序列分析结果证明，目的基因成功克隆入载体。 结论 成功构建了MN.CA9多糖蛋白表达载体。

    关键词 :宫颈癌;MN.CA9抗原;多糖蛋白;基因克隆

    Cloning and sequencing of proteoglycan protein cDNA of MN.CA9antigen in human cervical cancer

    ZHAO Jing

    (Department of Obstetrics and Gynecdogy，Tianjin General Hospital，Tianjin Medical University，Tianjin300052，China)

    Abstract:Objective To obtain the cDNA of proteoglycan protein of MN.CA9antigen of cervical cancer and construct the eukaryotic gene targeting vector pCA13-MN.Methods The total RNA was extracted from human cervical carcinoma cell line HeLa and the intact cDNA of proteoglycan protein was amplified by RT-PCR.The cDNA was cloned into pCA13vector and transformed into E.coli JM109.Results The result of sequencingwas completely consistentwith the published sequence of the proteoglycan protein cDNA.Conclusion The proteoglycan protein cDNA of MN.CA9antigen in human cervical cancer has been successfully cloned for preparing the tumor vaccine of MN.CA9antigen. ......