Glycogen increases in astrocytes in periª²intracerebral hemorrhage areas in rats

    LI Gaiª²Li1,ZHANG Shouª²Xin1,WANG Bingª²Ang2,WANG Baiª²Ren3

    1Department of Neurology,2Department of Orthopaedics,Third Hospital of PLA, Baoji 721004£¬China,3PLA Institute of Neurosciences,Fourth Military Medical University, Xi¬ðan 710033,China

    ¡¾Abstract¡¿ AIM: To study the ultrastructural changes around intracerebral hemorrhage (ICH) foci and to investigate the pathophysiological processes after ICH so as to provide some theoretical basis for clinical treatment.METHODS: Collagenase was injected into the striatum of the rats to cause bleeding and transmission electron microscope was used to observe the changes of the ultrastructure and the glycogen level in and around the pathological foci 24 h after ICH and the corresponding areas in controls.RESULTS: The electron microscopy observed a variety of fine structural changes in different kinds of cells around the pathological changes 24 h after ICH.The most significant finding was that large numbers of glycogen accumulated in astrocyte cytoplasm and their processes around the blood clot in brain.The number of glycogen granule was maximal in the processes of the astrocytes around the capillary vessels.There were no glycogen granule in microglial and oligodendroglial cells in foci.In the corresponding areas of the control,the ultrastructures were normal in all kinds of cells and no glycogen granule accumulated in neurons,neuroglial cells and endothelial cells in foci.CONCLUSION: Glycogen greatly increases around blood clot after ICH,suggesting that significant glucose metabolism disorder exists in foci. ......