γ干扰素对骨吸收及实体瘤骨转移的体外抑制作用
骨吸收,,],干扰素γ;肿瘤转移;骨吸收,1材料和方法,2结果,3讨论,[参考文献]
[摘要] 目的 探讨体外实验中γ干扰素(IFNγ)对其他细胞因子引起的骨吸收及实体瘤骨转移病人外周血单个核细胞培养上清(PBMCs)促进的骨吸收的抑制作用。方法 利用新生鼠头顶骨进行体外骨吸收实验,分别验证细胞因子白细胞介素1(IL1)、白细胞介素6(IL6)、肿瘤坏死因子(TNF)、IFNγ、粒巨噬细胞生长因子(GMCSF)在体外对骨吸收的影响。检测8例实体瘤骨转移阴性及11例骨转移阳性病人PBMCs的骨吸收活性(BRA)。结果 IL1、IL6、TNF、GMCSF均可促进体外骨吸收,以IL1作用最强。而上述因子联合培养,BRA明显增强。IFNγ本身对骨吸收作用不明显,但能使IL1及细胞因子联合培养引起的骨吸收减弱。IFNγ可使实体瘤骨转移阳性病人PBMCs诱发的BRA增强明显降低(t=2.34,P<0.05)。结论 IL1、IL6、TNF、GMCSF在体外对骨吸收有促进作用;IFNγ在体外可使部分细胞因子及实体瘤骨转移阳性病人PBMCs促进的骨吸收作用减弱,提示γ干扰素可能存在抑制肿瘤转移作用。[关键词] 干扰素γ;肿瘤转移;骨吸收
INHIBITORY EFFECT OF INFγ ON BONE RESORPTION AND BONE METASTASIS OF SOLID TUMOR IN VITRO
ZHANG MINGYONG, WANG HONGMEI,SUN WENXIN, et al
(Department of Respiratory Diseases, Qingdao Municipal Hospital, Qingdao 266071,China)
[ABSTRACT]ObjectiveTo explore the inhibitory effect of IFNγ on bone resorption stimulated by cytokines and peripheral blood mononuclear cells cultural supernatant (PBMCs) of solid tumor patients with bone metastasis.MethodsBone resorption test was carried out using neonatal mouse calvaria to investigate the bone resorbing activity (BRA) of cytokines (IL1, IL6, TNF, GMCSF, and IFNγ). The BRA of PBMCs of 19 cases of solid tumor patients with or without bone metastasis and its interaction with IFNγ were tested.ResultsIL1,IL6,TNF, and GMGSF could stimulate bone resorption, and IL1 was the most potent stimulator, but when these cytokines were cocultured, the BRA was significantly strengthened. IFNγ itself had no evident effect on bone resorption, but it could ameliorate bone resorption induced by other cytokines. IFNγ could inhibit the BRA induced by PBMCs of solid tumor patients with multiple bone metastasis significantly, as compared with the control group without IFNγ(t=2.34,P<0.05).Conclusion IFNγ could reduce the BRA strengthened by some cytokines and PBMCs of patients with bone metastasis of solid tumors, which suggests that IFNγ may inhibit bone metastasis in solid tumors. ......
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