抗生素诱导SPF级BALB/c小鼠胃肠道白念珠菌定植模型
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黄雪峰, 袁 静, 魏 泓
抗生素;白念珠菌定植;微生物区系,黄雪峰,袁静,魏泓,通讯作者,Aspecific-pathogen-freeBALB,cmousemodel
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黄雪峰, 西南大学生命科学学院 重庆市 400715
袁静, 魏泓, 中国人民解放军第三军医大学基础部动物学教研室 重庆市 400038
黄雪峰, 西南大学硕士研究生, 主要从事胃肠道微生物分子生态学的研究.
通讯作者: 魏泓, 400038, 重庆市, 中国人民解放军第三军医大学基础部动物学教研室. weihong63528@163.com
电话: 023-68752051 传真: 023-68752051
收稿日期: 2006-03-06 接受日期: 2006-03-24
A specific-pathogen-free BALB/c mouse model of Candida albicans gastrointestinal colonization following antibiotic treatment
Xue-Feng Huang, Jing Yuan, Hong Wei
Xue-Feng Huang, School of Life Science, Southwest University, Chongqing 400715, China
Jing Yuan, Hong Wei, Department of Animal Science, the Third Military Medical University, Chongqing 400038, China
Correspondence to: Hong Wei, Department of Animal Science, the Third Military Medical University, Chongqing 400038, China. weihong63528@163.com
Received: 2006-03-06 Accepted: 2006-03-24
Abstract
AIM: To establish a specific-pathogen-free (SPF) BALB/c mouse model of Candida albicans gastrointestinal colonization following antibiotic treatment.
METHODS: Female SPF BALB/c mice were treated for 5 days (120 h) with ceftriaxone ad libitum in drinking water, followed by a single oral gavage of 107CFU (50 µL) C. albicans. The gastrointestinal microbiota of mouse model was analyzed by plate counts and 16S rDNA-based polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) technique.
RESULTS: The culturable anaerobic and enteric bacterial levels were reduced significantly by 99.99% at day 5 of antibiotic treatment (anaerobe: 8.53 ± 0.31 Log10CFU/g vs 4.18 ± 0.90 Log10CFU/g,P < 0.01; enteric bacteria: 3.67 ± 0.14 Log10CFU/g vs 0, P < 0.01). DGGE profile clearly indicated that the bacterial diversity of the gut microbiota was obviously decreased (from 27-32 bands to 2-7 bands). A single oral gavage of 107CFU C. albicans resulted in C ......
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