美洛昔康对结肠癌细胞VEGF和angiopoietin-2表达的影响
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陶凯雄, 张 宁, 王国斌, 吴相柏
美洛昔康;结肠癌;环氧合酶2;血管内皮生长因子;血管生成素-2,陶凯雄,张宁,王国斌,吴相柏,陶凯雄,通讯作者,Effectsof
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陶凯雄, 张宁, 王国斌, 吴相柏, 华中科技大学同济医学院附属协和医院腹腔镜外科中心 湖北省武汉市 430022
陶凯雄, 医学博士, 外科学副教授、副主任医师, 硕士研究生导师, 华中科技大学同济医学院附属协和医院腹腔镜外科中心副主任, 主要从事消化道恶性肿瘤的靶向诊治以及微创治疗.
国家863计划, No. 2001AA22218051
湖北省科技攻关计划, No. 2005AA304B09
通讯作者: 陶凯雄, 430022, 华中科技大学同济医学院附属协和医院腹腔镜外科中心. kaixgtao@public.wh.hb.cn
电话: 027-85726405 传真: 027-85756343
收稿日期: 2006-01-26 接受日期: 2006-03-20
Effects of meloxicam on vascular endothelial growth factor and angiopoietin-2 expression in colon carcinoma cell line HT-29
Kai-Xiong Tao, Ning Zhang, Guo-Bin Wang, Xiang-Bai Wu
Kai-Xiong Tao, Ning Zhang, Guo-Bin Wang, Xiang-Bai Wu, Department of General Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China
Supported by the National High Technology Research and Development Program of China, No. 2001AA22218051, and the Key Science and Technology Research and Development Program of Hubei Province, No. 2005AA304B09
Correspondence to: Kai-Xiong Tao, Department of General Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China. kaixgtao@public.wh.hb.cn
Received: 2006-01-26 Accepted: 2006-03-20
Abstract
AIM: To investigate the effect of meloxicam, a nonsteroidal anti-inflammatory drug (NSAID), on the expression of vascular endothelial growth factor (VEGF) and angiopointin-2 (Ang-2) in colon carcinoma cell line HT-29.
METHODS: The cultured HT-29 cells were treated with different concentrations of meloxicam (100, 200, 400, 800 μmol/L) for different time. The proliferation of HT-29 cells was detected by Cell Counting Kit 8 (CCK8). Cell cycle was detected by flow cytometry. The levels of VEGF and Ang-2 protein in the supernatants were determined by enzyme-linked immunosorbent assay (ELISA) and the expression of VEGF and Ang-2 mRNA in HT-29 cells were examined by real-time quantitative reverse-transcription-polymerase chain reaction (RT-PCR) ......
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