EBV相关胃癌ras基因突变及法尼基转移酶β-亚单位基因的表达
http://www.100md.com
孙佰秀, 殷 凡, 朱 伟, 高 玉, 孙淑红,
胃肿瘤;EB病毒;PCR-RFLP;RT-PCR;ras;FTase,孙佰秀,殷凡,朱伟,高玉,孙淑红,罗兵,通讯作者,Rasmutat
 |
| 第5页 |
 |
| 第1页 |
参见附件(882KB，6页)。
孙佰秀, 青岛市妇女儿童医疗保健中心检验科 山东省青岛市 266011
殷凡, 朱伟, 高玉, 孙淑红, 罗兵, 青岛大学医学院微生物学教研室 山东省青岛市 266021
孙佰秀, 1995年青岛大学医学院本科毕业, 主管检验师, 主要从事临床检验工作.
通讯作者: 罗兵, 266021, 山东省青岛市登州路38号, 青岛大学医学院微生物教研室. qdluobing@yahoo.com
电话: 0532-83812423
收稿日期: 2006-02-06 接受日期: 2006-03-20
Ras mutation and expression of farnesyltransferase β mRNA in Epstein-Barr virus-associated gastric carcinoma
Bai-Xiu Sun, Fan Yin, Wei Zhu, Yu Gao, Shu-Hong Sun, Bing Luo
Bai-Xiu Sun, Clinical Laboratory, Qingdao Women & Children’s Medical and Health Care Center, Qingdao 266011, Shandong Province, China
Fan Yin, Wei Zhu, Yu Gao, Shu-Hong Sun, Bing Luo, Department of Microbiology, Qingdao University Medical College, Qingdao 266021, Shandong Province, China
Correspondence to: Dr. Bing Luo, Department of Microbiology, Qingdao University Medical College, 38 Dengzhou Road, Qingdao 266021, Shandong Province, China. qdluobing@yahoo.com
Received: 2006-02-06 Accepted: 2006-03-20
Abstract
AIM: To investigate ras gene mutations and farnesyltransferase (FTase) β mRNA expression in Epstein-Barr virus(EBV)-associated gastric carcinoma (EBVaGC), EBV-negative gastric carcinoma (EBVnGC) with matched clinico-pathological parameters and corresponding adjacent tissues of gastric carcinoma.
METHODS: The mutations of ras gene were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and the expression of FTase β mRNA was tested by reverse transcription PCR (RT-PCR) in 13 EBVaGCs, 45 EBVnGCs with matched clinicopathological parameters and 58 corresponding adjacent tissues of gastric carcinoma.
RESULTS: H-ras mutation at codon 12 was detected in 2(3.45%) cases of gastric carcinomas, both of which were EBVnGCs. No K-ras mutations occurred at codon 12 and 13 in all cases. The level of FTase β mRNA expression was 0 ......
您现在查看是摘要介绍页，详见PDF附件(882KB，6页)。