食管鳞癌细胞系EC-109中缺氧诱导因子-1α的表达意义
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杜琰萍, 施瑞华, 肖 斌, 朱 宏, 凌亭生,
缺氧;缺氧诱导因子-1α;食管鳞癌;基因表达调控,杜琰萍,施瑞华,肖斌,朱宏,凌亭生,张国新,郝波,杜琰萍,通讯作者,Expressionan
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杜琰萍, 施瑞华, 肖斌, 朱宏, 凌亭生, 张国新, 郝波, 南京医科大学第一附属医院消化科 江苏省南京市 210029
杜琰萍, 南京医科大学第一附属医院消化科在读硕士.
江苏省自然科学基金资助项目, No. BK2005153
通讯作者: 施瑞华, 210029, 江苏省南京市, 南京医科大学第一附属医院消化科. ruihuashi@126.com
电话: 025-83718836-6032
收稿日期: 2006-02-28 接受日期: 2006-03-15
Expression and significance of hypoxia-inducible factor-1 alpha in esophageal squamous cancer cell line EC-109
Yan-Ping Du, Rui-Hua Shi, Bin Xiao, Hong Zhu, Ting-Sheng Ling, Guo-Xin Zhang, Bo Hao
Yan-Ping Du, Rui-Hua Shi, Bin Xiao, Hong Zhu, Ting-Sheng Ling, Guo-Xin Zhang, Bo Hao, Department of Gastroenterology, the Frist Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China
Supported by the Natural Science Foundation of Jiangsu Province, No. BK2005153
Correspondence to: Professor Rui-Hua Shi, the Frist Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China. ruihuashi@126.com
Received: 2006-02-28 Accepted: 2006-03-15
Abstract
AIM: To investigate expression and significance of hypoxia inducible factor-1α (HIF-1α) in human esophageal squamous cancer cell line EC-109.
METHODS: Semiquantity reverse transcription-polymerase chain reaction (RT-PCR) and Western blot were performed to detect the expression of HIF-1α mRNA and protein in esophageal squamous cancer cell line EC-109 exposed to normal oxygen or hypoxia condition.
RESULTS: HIF-1α mRNA was over-expressed in esophageal squamous cancer cell line EC-109 under hypoxia condition (P < 0.05), and reached the peak at 24 h. However, HIF-1α protein had no significant up-regulation in hypoxia (P > 0.05).
CONCLUSION: Hypoxia can induce high expression of HIF-1α at mRNA level, but not at protein level.
Key Words: Hypoxia; Hypoxia inducible factor-1α; Esophageal squamous cell carcinoma; Gene expression regulation
Du YP, Shi RH, Xiao B, Zhu H, Ling TS, Zhang GX, Hao B ......
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