艾灸预处理对应激性溃疡大鼠胃黏膜HSP70蛋白及mRNA表达的影响
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常小荣, 彭 娜, 易受乡, 彭 艳, 严 洁
艾灸;足三里;梁门;应激性溃疡;热休克蛋白70;表达,常小荣,易受乡,严洁,彭娜,彭艳,常小荣,通讯作者,Effectsofmoxi
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常小荣, 易受乡, 严洁, 湖南中医药大学针灸推拿学院 湖南省长沙市 410007
彭娜, 彭艳, 湖南中医药大学针灸推拿学院2003级硕士 湖南省长沙市 410007
常小荣, 湖南中医学院教授, 针灸学教研室主任, 针灸重点学科学术带头人, 主要从事针灸经络及针灸治病机理的研究.
国家自然科学基金资助项目, No. 30572310
湖南省自然科学基金资助项目, No. 05JJ 40028
通讯作者: 常小荣, 410007, 湖南省长沙市韶山中路113号, 湖南中医药大学针灸推拿学院. xrchang1956@sina.com
电话: 0731-5381163 传真: 0731-5557891
收稿日期: 2006-02-28 接受日期: 2006-03-15
Effects of moxibustion on expression of HSP70 protein and mRNA in stress-induced gastric ulcer of rats
Xiao-Rong Chang, Na Peng, Shou-Xiang Yi, Yan Peng, Jie Yan
Xiao-Rong Chang, Na Peng, Shou-Xiang Yi, Yan Peng, Jie Yan, Acupuncture and Massage College, Hunan University of Traditional Chinese Medicine, Changsha 410007, Hunan Province, China
Supported by National Natural Science Foundation of China, No. 30572310, and the Natural Science Foundation of Hunan Province, No. 05JJ 40028
Correspondence to: Xiao-Rong Chang, Acupuncture and Massage College, Hunan University of Traditional Chinese Medicine, Changsha 410007, Hunan Province, China. xrchang1956@sina.com
Received: 2006-02-28 Accepted: 2006-03-15
Abstract
AIM: To explore the mechanism of moxibustion on the acupoints of Foot-Yangming Meridian in protecting gastric mucosa by observing the effects of moxibustion pretreatment at Zusanli (ST36) and Liangmen (ST21) on HSP70 protein and mRNA expression in the stress-induced gastric ulcer of rats.
METHODS: Sixty Sprague Dawley rats were averagely randomized into blank group (A), model group (B), Zusanli and Liangmen-moxibustion group (C) and control group (D). The expression of HSP70 protein and mRNA of gastric mucosa were determined by immunohistochemistry and reverse transcriptase polymerase chain reaction (RT-PCR). The content of endothelin (ET) and prostaglandin E2 (PGE2) in gastric mucosa were detected by radioimmunoassay ......
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