Genistein enhances cisplatin induced apoptosis of human hepatocellular cancer cell SMMCª²7721

    JIN Wei,MA Li

    Department of Gastroenterology,Second Affiliated Hospital,Lanzhou Medical College,Lanzhou 730030,China

    ¡¾Abstract¡¿ AIM: To investigate the inhibitory effects of genistein combined with cisplatin on the antiª²proliferation,apoptotic induction and expression of NFª²¦ÊB in human hepatocellular cancer cell SMMCª²7721.METHODS: After the respective treatment with genistein,cisplatin and genistein combined with cisplatin,the cell viability was examined by methyl thiazolyl tetrazolium assay.The morphology of cells was observed respectively under transmission electron microscope and laser scanning confocal microscope.The flow cytometry was used to detect the apoptosis rates and cell cycle distribution.The expressions of NFª²¦ÊB were determined by the immunocytochemical method.RESULTS: MTT showed that the inhibitory effect in the coª²treatment group was more obvious after exposure to drugs for 24 h and 48 h (A570 nm: 0ª±497¡À0ª±030¡ú0ª±295¡À0ª±098,0ª±791¡À0ª±042¡ú0ª±312¡À0ª±019,P<0ª±01). Microscopy showed that cells presented some morphologic characteristics of apoptosis.The flow cytometry indicated that after exposure to drugs,the apoptotic rates were higher in the coª²treatment group with genistein and cisplatin.The effect in the coª²treatment group showed a cell cycle blocking at G2/M phase.The immunocytochemical method showed that the expression levels of NFª²¦ÊB were overexpressed after exposure to cisplatin (64ª±9¡À5ª±4)%, while the downregulation of NFª²¦ÊB protein expression was seen in the coª²treatment group[(23ª±4¡À2ª±1)%,P<0ª±01].CONCLUSION: Genistein combined with cisplatin can significantly suppress the growth of hepatocellular cancer cells through induction of apoptosis,which associates with the downregulation of NFª²¦ÊB and may be useful for cancer chemotherapy. ......