MRSA__(期刊论文)

MRSAPBP2a蛋白的体外诱导、抗体制备及鉴定

http://www.100md.com 《第四军医大学学报》 2005年第9期

MRSA,,MRSA；PBP2a蛋白；体外诱导；动物免疫,1材料和方法,2结果,3讨论,【参考文献】

     Induction of expression of MRSAPBP2a protein in vitro and its antibody preparation

    SU MingQuan, YANG Liu, YUE QiaoHong, FAN Xin, HAO XiaoKe

    Department of Clinical Laboratory, Xijing Hospital, Fourth Military Medical University, Xian 710033, China

    【Abstract】 AIM: To induce the expression of MRSAPBP2a protein in vitro and to prepare its antibody against MRSAPBP2a. METHODS: Methicillinresistant Staphylococcus aureus (MRSA) were isolated by agar’s slides according to National Committee for Clinical Laboratory Standards (NCCLS) protocols and polymerase chain reaction (PCR). The expression of MRSAPBP2a was induced in vitro by addingLactam antibiotics. MRSAPBP2a was purified and was used to immune rabbits and the antiserum was identified by double immunodiffusion. RESULTS: Two strains of MRSA were isolated using the agar’s slides and PCR. SDSPAGE and Western blot analysis revealed the successful induction of PBP2a protein expression. Antibodies were detected in antiserum in 1∶32 and 1∶64 dilutions. CONCLUSION: MRSAPBP2a is induced to express in vitro and is purified successfully. The antibodies against MRSAPBP2a are prepared successfully, which lays the foundation for further study on MRSAPBP2a activities, MRSA immunotherapy and MRSA identification. ......

百拇医药网 http://www.100md.com/html/DirDu/2006/05/08/97/20/96.htm

您现在查看是摘要页，全文长 8219 字符。