当前位置: 首页 > 期刊 > 《第四军医大学学报》 > 2005年第7期
编号:10972713
PTTG反义cDNA对胆囊癌细胞5FU敏感性的影响
http://www.100md.com 《第四军医大学学报》 2005年第7期
胆囊肿瘤,,胆囊肿瘤;垂体肿瘤转化基因;氟尿嘧啶,1材料和方法,2结果,3讨论,【参考文献】
     PTTG antisense cDNA enhances sensitivity of gallbladder cancer cells to 5FU

    DOU GuoRui1, WANG Jiang2, ZHANG FuQin2

    1Brigade of Cadets, 2Department of Hepatobiliary Surgery, Xijing Hospital, Fourth Military Medical University, Xian 710033, China

    【Abstract】 AIM: To investigate the effect of PTTG antisense cDNA on the sensitivity of gallbladder cancer cells to 5FU. METHODS: The recombinant vector containing PTTG antisense cDNA was transfected into GBCSD gallbladder cancer cells. The positive cell clones were screened by G418. PTTG protein expression was detected by Western blot analysis. The changes of the cell proliferation and cell relative viability after treatment with different concentrations of 5FU were assessed by MTT, the doseeffects curves were obtained and IC50s were calculated. Flow cytometry was used to demonstrate discrepancy of apoptotic rates. RESULTS: The expression of PTTG protein in transfected cells was decreased significantly as compared with that in nontransfected ones. The cell proliferation was accelerated and the cell apoptotic rate was higher in transfected cells. The IC50 of 5FU was significantly lower in transfected cells (0.605 mmol/L) than in nontransfected and empty vector transfected cells (1.117 mmol/L and 0.914 mmol/L respectively). After treatment with 5FU, the apoptotic ratio was significantly higher in transfected cells (26.24%) than in nontransfected and empty vector transfectedcells (2.67% and 7.62% respectively). CONCLUSION: PTTG antisense cDNA transfection enhances the sensitivity of gallbladder cancer cells to 5FU. Such an enhancement of sensitivity suggests that the combination of PTTG antisense cDNA with 5FU can be an effective treatment modality for gallbladder cancer. ......

您现在查看是摘要页,全文长 12049 字符