心肌_细胞学_____大鼠；心肌(期刊论文)

成年SD大鼠心肌细胞的培养

http://www.100md.com 《第四军医大学学报》 2005年第8期

心肌,细胞学,,大鼠；心肌,细胞学；逆行灌流；细胞，培养的,1材料和方法,2结果,3讨论,【参考文献】

     Isolation and culture of adult SD rat cardiomyocytes

    ZHAI YingJiu,DONG XueHong,ZHOU LiNuo,HU RenMing

    Department of Endocrinology,Institute of Endocrine ＆ Diabetes,Huashan Hospital,Medical College,Fudan University,Shanghai 200040，China

    【Abstract】 AIM: To establish a stable method to isolate and culture adult SD rat cardiomyocytes.METHODS: The adult SD rat cardiomyocytes were isolated by perfusing retrogradely via the aorta and then cultured in vitro.The morphological changes of cardiomyocytes were observed.RESULTS: Freshly isolated cells were rodshaped and striated,beating asynchronously.The cells cultured under the condition with fetal calf serum(FCS)changed rapidly in morphology and presented dedifferentiation ......

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