Relationship between hypoxicª²endurance and erythropoietin in ovarian carcinoma cell line

    LIU Xiaoª²Feng1, CHEN Biª²Liang1, MA Xiaoª²Dong2, MA Xiangª²Dong1, WANG Deª²Tang1

    1Department of Obstetrics and Gynecology, Xijing Hospital, Fourth Military Medical University, Xi¬ðan 710033, China, 2Department of Obstetrics and Gynecology, PLA 537th Hospital, Baoji 721006, China

    ¡¾Abstract¡¿ AIM: To investigate the endurance of ovarian carcinoma cell line to hypoxia by measuring the expression of erythropoietin (EPO) and to study the role of EPO in the antiª²apoptosis and the genesis and development of ovarian carcinoma. METHODS: The 3AO cells plated in culture bottles were placed in an anaerobic system consisting of 950 mL/L N2 and 50 mL/L CO2 for different time periods. The cell growth was observed by MTT assay, the apoptosis rate was detected by FCM and the mRNAs expression of EPO was detected by in situ hybridization. RESULTS: Apoptosis of cells cultured under hypoxic condition increased after 6 h of hypoxic exposure compared with that at 0 h (P<0.01) and the activity of cell growth decreased obviously. The intensity of expression of EPOª²mRNA increased from 2 h after hypoxic exposure. The number of apoptosis cells reduced after 16 h of hypoxic exposure and the activity of cell growth gradually resumed. After 24 h of hypoxic culture, apoptosis was the same as that at 0 h. CONCLUSION: EPO is correlated with hypoxicª²endurance and it may be involved in antiª²apoptosis of ovarian cancer cells under hypoxic condition. ......