过氧化氢酶__(期刊论文)

过氧化氢酶对SW480细胞应激反应的影响

http://www.100md.com 《第四军医大学学报》 2005年第8期

过氧化氢酶,,过氧化氢酶；SW480细胞；细胞凋亡;细胞因子类;NFkB,1材料和方法,2结果,3讨论,【参考文献】

     Effects of catalase on stress reaction of SW480 cells

    WANG QunYing,WANG JiDe,ZHONG ShiShun,LI LiangRen,LAI ZhuoSheng,ZHANG ZhenShu,ZHANG YaLi

    Department of Digestive Diseases,Nanfang Hospital,Nanfang Medical University,Guangzhou 510515,China

    【Abstract】 AIM： To investigate the effects of catalase (CAT) on the expression of cytokines and the activation of nuclear factor kB in intestinal epithelial cells SW480.METHODS： SW480 cells were stimulated by LPS (Liposaccharide).The apoptosis of cells was detected by flow cytometry.The expressions of TNF α,IL1β and IL8 in the cells were detected by semiquantitative reverse transcription polymerase chain reaction (RTPCR).The activation of NFκB in the cells was evaluated by electrophoretic mobility shift assay (EMSA).RESULTS： Compared with the cells treated by LPS(3967±612)% alone,the apoptosis(2497±235)% decreased in the cells pretreated with CAT.Furthermore,the expressions of TNFα (065±059), IL1β (032±006)and IL8 (062±025) were significantly decreased (P<005).The activation of NFκB was also suppressed.CONCLUSION： CAT can reduce the reaction of stress SW480 cells,due to the suppression of NFκB activity . ......

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