Effect of bFGF on VEGF expression and biological behavior of marrow stromal cells

    CHANG Qi£¬ LIU Jian£¬ HU Yunª²Yu£¬ MENG Guoª²Lin, BAI Jianª²Ping£¬ PENG Lei

    Institute of Orthopedics of Chinese PLA, Xijing Hospital, Fourth Military Medical University, Xi¡¯an 710033, China

    ¡¾Abstract¡¿ AIM: To investigate the effects of basic fibroblast growth factor (bFGF) on vascular endothelial growth factor (VEGF) expression and the biological behavior of marrow stromal cells. METHODS: The marrow stromal cells of the femur of rabbits were procured and cultured. The samples were treated with different doses of bFGF and the control was not treated. Five days later, the effects were observed by histopathological analysis, cells multiplication, alkaline phosphatase activities detection, the computer analysis of bone clusters and the detection of ratio of VEGFª²positive cells. RESULTS: There was statistically significant difference between different dose groups in all the detection items and the results of treatment groups were all better than that of the control group. CONCLUSION: bFGF can stimulate both the multiplication and osteogenic potential of marrow stromal cells in a dose dependent manner and the best dose of bFGF is 1200 kU¡¤L-1. ......