Effects of salidroside on expression of Smads gene in rats with hepatic fibrosis

    WU Xiaoª²Ling1,ZENG Weiª²Zheng2,JIANG Mingª²De2,WANG Piª²Long1£¬DENG Guiª²Ying3£¬QIN Jianª² Ping2

    1Department of Gastroenterology£¬First Affiliated Hospital of Chongqing University of Medical Sciences£¬Chongqing 400016£¬China£¬2Department of Gastroenterology£¬General Hospital of Chengdu Military Command,Chengdu 610083£¬China£¬3Department of Gastroscope,Sixth Hospital of Chengdu City£¬Chengdu 610051£¬China

    ¡¾Abstract¡¿ AIM: To observe the effects of salidroside on the expression of Smads gene in rats with CCl4ª²induced hepatic fibrosis and to investigate its probable molecular mechanisms in interfering liver fibrosis.METHODS: Hepatic fibrosis in rats was induced by subcutaneous injection of CCl4.The expressions of Smads mRNA were detected with in situ hybridization (ISH).The level of Smads protein in the liver tissue was detected with immunohistochemistry(IH) and the deposition of collagen in the liver was observed with HE and Masson staining.RESULTS: The content of collagen obviously increased in the liver of model group and the expression of Smad 4 mRNA in fibrotic liver was significantly enhanced.The positive ratio of Smad 4 protein significantly increased.All these increases were significantly decreased with salidroside treatment.However,the expression of Smad 7 in model group was very feeble but salidroside enhanced it obviously.CONCLUSION: Salidroside is effective in protecting rat liver against CCl4ª²induced hepatic fibrosis perhaps by interfering the gene expression of Smads,which was essential in the fibrogenisis process of liver. ......