摘要:目的 探讨兔严重烫伤(50%Ⅲ度)早期血脑屏障开放的最早时间、部位及特征。 方法 雄性大白兔42只，随机分成6组(对照组、烫伤后1、2、2.5、3、4小时组)，烫伤组均制成50%Ⅲ度烫伤模型。采用125 I标记的牛血清白蛋白(125 I-BSA)作为示踪剂，并与各组电镜结果进行对比观察。 结果 125 I-BSA的在体实验证实，烫伤后2.5小时双侧前叶、小脑的比放射性较对照组有显著性增加。电镜结果表明烫伤后2小时，双侧小脑、前叶皮质部分毛细血管周围出现水肿。 结论 兔50%Ⅲ度烫伤后2小时，双侧前叶和小脑处血脑屏障出现开放;烫伤后3～4小时，脑组织出现弥漫性血脑屏障开放。125 I-BSA测定微血管通透性灵敏度高，可精确地定量分析，全面、确切地反映早期血脑屏障的病理变化。

    关键词: 严重烫伤;血脑屏障; 125 I标记的牛血清白蛋白(125 I-BSA);兔

    Dynamic research of the opening of blood-brain barrier in the early stage of severely scalded rabbits

    HU Jun，LI Hai-tao，DING Shi-yi，et al.

    (Department of Radiology，Southwestern Hospital，Third Military Medical University，Chongqing 400038，China)

    Abstract: Objective To explore the earliest time，position and characteristics of the opening BBB in the early stages of severely scalded rabbits.Methods Forty-two rabbits were randomized into control、1、2、2.5、3and4h groups after scald injury.The rabbits in wounded groups were inflicted with50%TBSA ofⅢdegree skin scald.125 I labeled bo-vine serum albumin(125 I-BSA)was used as tracer to observe the earliest time point and position of opening of BBB after severe scald.The results were compared with the changes of ultrastructure by electronic microscopic.Results 125 I-BSA intake began to increase remarkably at2.5hour in anterior cortex and cerebellum ......