一种新的阿片肽对小鼠脑和小肠组织阿片受体和肽转运载体基因的影响
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韩 飞, 施用晖, 乐国伟, 郑建良, 刘 勇
新阿片肽;小鼠;脑;小肠组织;阿片受体;肽转运载体;基因转录韩飞,施用晖,乐国伟,郑建良,刘勇.一种新的阿片肽对小鼠脑和小肠组织阿片受体和肽转运载体基因的影响. 世界华人消化杂志2006;
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韩飞,施用晖, 乐国伟, 郑建良,刘勇, 江南大学食品学院, 江南大学工业生物技术教育部重点实验室 江苏省无锡市 214036
韩飞, 博士,现在国家粮食局科学研究院工作, 主要从事粮食营养与安全研究.
中央级科研院所基础性工作专项资金重点资助项目, No. 2100DEA 20022
通讯作者: 乐国伟, 214036, 江苏无锡市惠河路170号, 江南大学食品学院营养与生物技术教研室. lgw@sytu.edu.cn
电话: 0510-5869236
收稿日期: 2005-03-18 接受日期: 2005-10-09
Effect of a new opioid peptide on opioid receptor and peptide transporter gene of brain and small intestinal tissues in mice
Fei Han, Yong-Hui Shi, Guo-Wei Le, Jian-Liang Zheng, Yong Liu
Fei Han, Yong-Hui Shi, Guo-Wei Le, Jian-Liang Zheng, Yong Liu, Food Science and Technology School; Key Laboratory of Biotechnology, Southern Yangtze University, Wuxi 214036, Jiangsu Province, China
Supported by the Special Fund for the Key Project of Central Academy Basic Research, No. 2100DEA 20022
Correspondence to: Guo-Wei Le, Food Science and Technology School, Department of Biotechnology, Southern Yangtze University, Huihe Road 170, Wuxi 214036, Jiangshu province, China. lgw@sytu.edu.cn
Received: 2005-03-18 Accepted: 2005-10-09
Abstract
AIM: To investigate the effect of a new opioid peptide, YPFPGPIRYG, on the opioid receptor and peptide transporter gene of brain and small intestinal tissues via gastrointestinal tracts at transcription level.
METHODS: Twenty-four mice were randomly divided into opioid group (n = 12) and control group (n = 12), which were fed with 8×10-7 mol/L YPFPGPIRYG and double distilled water, respectively, for 2 wk. The total RNA was extracted from the brain and small intestinal tissues of mice for the detection ofm-,d-opioid receptor and peptide transporters PepT1 gene expression by reverse transcription-polymerase chain reaction (RT-PCR).
RESULTS: In the brain and small intestinal tissues, m- and d-opioid receptor transcripts were detected, while peptide transporter PepT1 was only found to be expressed in the small intestine ......
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