Protective effect of inosine on intrastriatal kianic acid injection induced lesion in the rat

    FENG Guoª²Dong, YOU Siª²Wei, JU Gong

    Institute of Neurosciences of Chinese PLA, Fourth Military Medical University, Xi¬ðan 710033,China

    ¡¾Abstract¡¿ AIM: To establish the animal model of intrastriatal kainic acid (KA) injection and to investigate the effect of systematic administration of inosine on the striatal lesion size and the number of NADPHª²d positive neurons in this model. METHODS: KA was stereotaxically injected into the left striatum of rats. Ten rats were divided into 2 groups: Inosine group (n=5), receiving ip inosine administration, (50 g¡¤L-1, 75 mg¡¤kg-1) , 3 times each day, from 1 day prior to operation to day 3 postoperatively when the rats were sacrificed, and control group (n=5), receiving normal saline instead of inosine. Nissl stain and NADPHª²d histochemistry were then performed to investigate the difference of lesion size and NADPHª²d positive neurons between inosineª² and salineª² treated groups. RESULTS: Inosine decreased the size of KAª²induced striatal lesion area and increased the number of NADPHª²d positive neurons. CONCLUSION: Inosine can attenuate KAª²induced striatal lesion and show protective effect on NADPHª²d positive neurons in the striatum. These findings provide a pontential clinical use of inosine in the CNS disease. ......