【摘要】 目的 重组表达人OGDC-E2融合蛋白，并用于检测自身抗体。方法 重组表达的OGDC-E2，分别采用免疫印迹法(IBT)法和酶联免疫吸附试验(ELISA)方法检测PBC患者的M2抗体。50例确诊的PBC患者为PBC组，以其他肝病患者70例、自身免疫病患者70例和健康体检者70例作对照。结果 50例确诊的PBC患者中41例OGDC-E2抗体阳性，9例阴性，阳性率为82.0%。而疾病对照组和健康体检者血清中M2抗体检测均为阴性。结论 用重组人2-氧戊二酸脱氢酶复合物E2亚单位检测M2抗体，有较好的敏感性及一定的特异性，有助于临床对PBC的诊断。

    【关键词】 2-氧戊二酸脱氢酶复合物E2亚单位；原发性胆汁性肝硬化；免疫印迹法；酶联免疫吸附试验；自身抗体

    Detection of anti-M2 autoantibody using recombinant OGDC-E2

    CHEN Yan,YAO Ding-kang,ZHOU Ye,et al.Labortary

    Diagnostics,Changzheng Hospital,The Second Military Medical

    University,and Clinical Immunology Center of PLA,Shanghai 200003,China

    【Abstract】 Objective To detect the level of anti-M2 autoantibody using recombinant OGDC-E2.Methods We purified recombinant OGDC-E2 by Ni2+ affinity chromatography column and then established Western blotting test(WBT) and enzyme linked immunosorbent assays (ELISA) to detect anti-M2 autoantibody in the sera of patients with primary biliary cirrhosis(PBC).Results Among 50 sera from PBC patients,41 were positive,all of controls were negative.Conclusion The recombinant OGDC-E2 could be used to detect anti-M2 autoantibody specially and sensitively,helpful for diagnosis of PBC. ......