关键词:补阳还五汤；缺血性中风后遗症；神经前体细胞；神经增殖

    摘要 目的：观察补阳还五汤对缺血性中风后遗症大鼠神经增殖作用的影响。方法：采用机械开颅电凝法阻断大脑中动脉(MCA)24 h后，用随机电脉冲刺激仪每天刺激2 h，连续刺激20 d造成大鼠缺血性中风后遗症模型。治疗组在造模结束后灌服补阳还五汤水煎液, 连续给药15 d。给药结束后处死大鼠，并取出大脑，处死前均腹腔注射5-溴脱氧核苷尿嘧啶(BrdU)，用免疫组织化学方法检测大鼠脑组织中BrdU的表达。结果：与模型组相比，补阳还五汤治疗组BrdU阳性细胞数量显著增多。结论：补阳还五汤对缺血性中风后遗症大鼠神经增殖具有促进作用。

    基金项目：国家自然科学基金(30371774)

    Supported by National Natural Science Foundation of China(30371774)

    作者简介：谭县辉(1978-)，男，在读硕士研究生，电话：020-61648265，E-mail：tfeihua@163.com

    通讯作者：佟 丽，研究员，硕士导师，电话：020-61648539, E­-mail:tli_88@yahoo.com

    Effects of Buyanghuanwu decoction on nerve proliferation in rats with ischemic stroke sequelae

    TAN Xian-hui¹, QU Hong-da¹, PENG Kang¹, CHEN Yu-yao¹, TONG Li¹, SHEN Jian-gang², ZHU Chuan-wu<sup>1

    1</sup>College of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China; ²School of Traditional Chinese Medicine, Chinese University of Hong Kong, Hong Kong, China

    Abstract: Objective To examine the effect of Buyanghuanwu decoction (BYHWD) in inducing nerve proliferation in rats with ischemic stroke sequelae. Methods A rat model of ischemic stroke sequelae was established by means of craniectomy in which the right common carotid artery was ligated with 4-0 silk thread followed by cauterization of the right middle cerebral artery. Programmed electric shock was administered 24 h after the onset of ischemic stroke for 2 h daily for 20 consecutive days. The rats in sham operation group were not subjected to ligation of the right common carotid artery or right middle cerebral artery occlusion. The rats in the treatment groups were given oral BYHWD for 15 consecutive days. All rats received repeated intraperitoneal injections of the cell proliferation-specific marker 5-bromodeoxyuridine (BrdU), and the intake of BrdU in the cerebral tissues was determined by immunohistochemistry. Results The number of BrdU-immunoreactive cells in the cerebral tissues of BYHWD-treated rats was significantly greater than that of the untreated model group. Conclusion BYHWD can promote nerve proliferation in rats with ischemic stroke sequelae. ......