关键词：柴胡总皂甙；戊四氮；癫痫；慢性点燃；脑电图

    摘要：目的 评价柴胡有效成分中柴胡总皂甙对戊四氮(PTZ)慢性点燃大鼠痫性发作及脑电图的影响。 方法 将48只健康SD大鼠随机均分为6组，即空白组、生理盐水组、丙戊酸钠(VPA)组和柴胡总皂甙高、中、低3种剂量组，除空白组不做处理外，其他组采用腹腔注射PTZ进行慢性点燃造模，造模同时给予以上不同处理因素，连续4周，在此期间记录大鼠痫性发作级别及次数，最后描记大鼠脑电图。 结果 柴胡总皂甙高、中、低3个剂量组在实验2周时可以降低大鼠的痫性发作率，其中以高剂量组显著(P<0.05)，柴胡皂甙高剂量组应用4周时能明显降低PTZ慢性点燃大鼠的点燃率(P<0.05)，并明显降低痫性发作级别(P<0.01)；脑电图也存在一定差异。 结论 柴胡总皂甙可拮抗PTZ慢性点燃大鼠的痫性发作，并有一定的对抗PTZ慢性点燃作用。

    Effect of saikosaponins on epileptic seizure and EEG in pentetrazole-induced kindling rats

    XIE Wei¹, BAO Yong¹, YU Li-jian¹, HOU Guang-nan², TAN Hong-xiang<sup>3

    1</sup>Department of Internal Medicine, School of Traditional Chinese Medicine, ²Center of EEG, Department of Neurology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China; ³Department of Neurological Rehabilitation, Guangzhou Children’s Hospital, Guangzhou 510120, China

    Abstract: Objective To study the effect of saikosaponins, the active ingredients of Bupleurum chinense DC., on epileptic seizure and EEG of pentetrazole (PTZ)-induced kindling rats. Methods Forty-eight healthy Sprague-Dawley rats were randomly divided into 6 equal groups, namely the blank control group, normal saline (NS) group, sodium valproate (VPA) group, and 3 saikosaponins groups of high, medium and small doses. Except those in the blank control group, the rats in the other groups were all given different treatments as specified prior to intraperitoneal PTZ injection to induce kindling on a daily basis for 4 consecutive weeks. Epileptic seizures of the rats were recorded during the treatment and EEG recorded at the end of the treatments. Results Seizure frequency in the 3 saikosaponins groups decreased 2 weeks later, which was especially obvious in the high-dose group (P<0.05). The rate of kindling was significantly lower in high-dose saikosaponins group than in the other treatment groups after 4 weeks of treatment (P<0.05), with also less intense seizure onset (P<0.01) and differences in the wave form of EEG. Conclusion Saikosaponins can inhibit PTZ-induced epileptic seizure in rats and antagonize the kindling effect of PTZ . ......