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白玉杰，Seetha SV， Vamla B, 高
乳腺肿瘤,,乳腺肿瘤；基因，抑制，肿瘤；BRCA2；酵母双杂交；FHL2,1材料和方法,2结果,3讨论,【参考文献】
Interaction between BRCA2 and the LIM domain only protein FHL2
BAI YuJie1, S Seetha2, BAND Vamla2， GAO QingShen2
1Institute of Genetic Diagnosis of Chinese PLA, Administration of Scientific Research, Fourth Military Medical University, Xian 710033, China, 2Department of Radiation Oncology, Medical School, Tufts University, Boston MA02100, USA
【Abstract】 AIM: To screen genes encoding proteins which can interact with BRCA2 via yeast twohybrid system, confirm the interaction and analyze their functional linkage. METHODS: 3′region of BRCA2 gene was used to construct the “bait” plasmid and to screen the cDNA library of normal human mammary gland. Coimmunoprecipitation, mammalian twohybrid and Luciferase assay were used to analyze the association and putative function. RESULTS: Many genes encoding proteins interacting with BRCA2, including the FHL2, were pulled out by yeast twohybrid screening. The in vivo binding and functional relation between BRCA2 and FHL2 was demonstrated by coimmunoprecipitation, mammalian twohybrid assay and luciferase detection. CONCLUSION: The interaction and the functional linkage between BRCA2 and FHL2 was confirmed and which provides fresh insights into the biological functions of BRCA2. ......
BAI YuJie1, S Seetha2, BAND Vamla2， GAO QingShen2
1Institute of Genetic Diagnosis of Chinese PLA, Administration of Scientific Research, Fourth Military Medical University, Xian 710033, China, 2Department of Radiation Oncology, Medical School, Tufts University, Boston MA02100, USA
【Abstract】 AIM: To screen genes encoding proteins which can interact with BRCA2 via yeast twohybrid system, confirm the interaction and analyze their functional linkage. METHODS: 3′region of BRCA2 gene was used to construct the “bait” plasmid and to screen the cDNA library of normal human mammary gland. Coimmunoprecipitation, mammalian twohybrid and Luciferase assay were used to analyze the association and putative function. RESULTS: Many genes encoding proteins interacting with BRCA2, including the FHL2, were pulled out by yeast twohybrid screening. The in vivo binding and functional relation between BRCA2 and FHL2 was demonstrated by coimmunoprecipitation, mammalian twohybrid assay and luciferase detection. CONCLUSION: The interaction and the functional linkage between BRCA2 and FHL2 was confirmed and which provides fresh insights into the biological functions of BRCA2. ......