Association of mycoplasma and immune factors with recurrent spontaneous abortion and sterility

    ZHAO Xiaoª²Lan1£¬ CHU Yongª²Lie2£¬ YE Guoª²Ling1£¬ CAI Xiaoª²Ning1£¬ LIU Qi1

    1Labratory of Genetics, Second Hospital, Xi¬ðan Jiaotong University, Xi¬ðan 710004£¬ China, 2Department of Pathobiology, Medical College, Xi¬ðan Jiaotong University, Xi¬ðan 710061, China

    ¡¾Abstract¡¿ AIM: To elucidate the association of recurrent spontaneous abortion (RSA) with mycoplasma infection and anticardiolipin antibodies (ACA) , and the relationship of sterility to mycoplasma infection and antisperm antibodies (ASA). METHODS: Ureaplasma urealyticum (UU) and mycoplasma hominis (MH) from the cervical secretions were detected respectively by culture method in 338 women who had the history of recurrent spontaneous abortion three times or more and 248 sterile women, which were assigned as monitoring group, and 105 normal women, who did not have any miscarriage and delivered a live child as control group. Enzymeª²linked immunoassay (ELISA) was carried out to detect both isotypes of ACA and ASA , immunoglobulin M (IgM) and immunoglobulin G (IgG). The positivity rate of each index between 2 groups was compared. RESULTS: The positivity rates of UU and MH in monitoring group was significantly higher than that in control group (P<0.05). The positivity rate of ACAª²IgM and ASAª²IgM in the monitoring group was significantly higher than that in the control group (P<0.05). For RSA patients, the positivity rate of ACAª²IgM, ACAª²IgG andACAª²IgM+ACAª²IgG in UU or MH positive group was significantly higher than that in UU and MH in negative group (P<0.05). For sterile women, the positivity rate of ASAª²IgM, ASAª²IgG in UU or MH positive group was significantly higher than that UU and MH in negative group (P<0.05). CONCLUSION: It is suggested that UU or MH infection is closely correlated with RSA and infertility; RSA is correlated with ACA; sterility is correlated with ASA; both ACA and ASA are correlated with UU or MH infection. ......